Columbia Center for Translational Immunology (CCTI)

Configuration Charts

BD Fortessa (CCTI Site)

Blue 488nm

  • Detector: A
    • Band Pass Filter: 780/60
    • Dichoric Filter: 750LP
    • Color name available on the instrument: PE-Cy7
    • Channel in LSRII: Yes
  • Detector: B
    • Band Pass Filter: 710/50
    • Dichoric Filter: 685LP
    • Color name available on the instrument: PerCP-Cy5.5/PerCP/PECy5
    • Additional Color: PI/7AAD
    • Channel in LSRII: Yes
  • Detector: C
    • Band Pass Filter: 610/20
    • Dichoric Filter: 600LP
    • Color name available on the instrument: PE-Texas Red
    • Additional Color: PI/7AAD/Fixable Red/PE-CF594
    • Channel in LSRII: Yes
  • Detector: D
    • Band Pass Filter: 575/26
    • Dichoric Filter: 550LP
    • Color name available on the instrument: PE
    • Additional Color: Sytox Orange/PI
    • Channel in LSRII: Yes
  • Detector: E
    • Band Pass Filter: 530/30
    • Dichoric Filter: 505LP
    • Color name available on the instrument: FITC/Alexa488
    • Additional Color: Sytox Green/Fixable Green/GFP
    • Channel in LSRII: Yes
  • Detector: F
    • Band Pass Filter: 488/10
    • Dichoric Filter: Blank
    • Color name available on the instrument: SSC
    • Channel in LSRII: ​​​​Yes

Violet 405nm

  • Detector: A
    • Band Pass Filter: 780/60
    • Dichoric Filter: 750LP
    • Color name available on the instrument: BV786
    • Channel in LSRII: No
  • Detector: B
    • Band Pass Filter: 710/50
    • Dichoric Filter: 690LP
    • Color name available on the instrument: BV711
    • Channel in LSRII: No
  • Detector: C
    • Band Pass Filter: 660/20
    • Dichoric Filter: 630LP
    • Color name available on the instrument: BV650
    • Channel in LSRII: Yes
  • Detector: D
    • Band Pass Filter: 610/20
    • Dichoric Filter: 595LP
    • Color name available on the instrument: BV605
    • Channel in LSRII: Yes
  • Detector: E
    • Band Pass Filter: 525/50
    • Dichoric Filter: 505LP
    • Color name available on the instrument: V500/BV510
    • Additional Color: CFP/Sytox Blue/Fixable Aqua/(BV570 filter change)
    • Channel in LSRII: Yes
  • Detector: F
    • Band Pass Filter: 450/50
    • Dichoric Filter: Blank
    • Color name available on the instrument: Pac Blue/V450/BV421
    • Additional Color: DAPI/Sytox Blue/Fixable Violet
    • Channel in LSRII: Yes

Red 633nm

  • Detector: A
    • Band Pass Filter: 780/60
    • Dichoric Filter: 750LP
    • Color name available on the instrument: APC-Cy7/APC-H7
    • Additional Color: Fixable Near IR/APC-A780
    • Channel in LSRII: Yes
  • Detector: B
    • Band Pass Filter: 730/45
    • Dichoric Filter: 710LP
    • Color name available on the instrument: Alexa700
    • Additional Color: APC-R700
    • Channel in LSRII: Yes
  • Detector: C
    • Band Pass Filter: 670/14
    • Dichoric Filter: Blank
    • Color name available on the instrument: APC
    • Additional Color: Sytox Red/Fixable Far Red/Alexa633/Alexa647/DRAQ5
    • Channel in LSRII: Yes

BD Fortessa (HICCC Site)

Blue 488nm

  • Detector: A
    • Band Pass Filter: 710/50
    • Dichoric Filter: 685LP
    • Color name available on the instrument: PerCP-Cy5.5
  • Detector: B
    • Band Pass Filter: 530/30
    • Dichoric Filter: 505LP
    • Color name available on the instrument: FITC
    • Additional Color: Sytox Green/Fixable Green/GFP/Alexa488
  • Detector: C
    • Band Pass Filter: 488/10
    • Dichoric Filter: Blank
    • Color name available on the instrument: SSC

Green 561nm

  • Detector: A
    • Band Pass Filter: 780/60
    • Dichoric Filter: 750LP
    • Color name available on the instrument: PE-Cy7
  • Detector: B
    • Band Pass Filter: 710/50
    • Dichoric Filter: 685LP
    • Color name available on the instrument: PE-Cy5.5
  • Detector: C
    • Band Pass Filter: 670/30
    • Dichoric Filter: 635LP
    • Color name available on the instrument: PE-Cy5
  • Detector: D
    • Band Pass Filter: 610/20
    • Dichoric Filter: 600LP
    • Color name available on the instrument: PE-Texas Red
    • Additional Color: 7AAD/PI/Fixable Red/PE-CF594
  • Detector: E
    • Band Pass Filter: 582/12
    • Dichoric Filter: Blank
    • Color name available on the instrument: PE
    • Additional Color: Sytox Orange/PI

Violet 405nm

  • Detector: A
    • Band Pass Filter: 780/60
    • Dichoric Filter: 750LP
    • Color name available on the instrument: BV786
  • Detector: B
    • Band Pass Filter: 710/50
    • Dichoric Filter: 690LP
    • Color name available on the instrument: BV711
  • Detector: C
    • Band Pass Filter: 660/20
    • Dichoric Filter: 630LP
    • Color name available on the instrument: Qdot655
    • Additional Color: BV650
  • Detector: D
    • Band Pass Filter: 610/20
    • Dichoric Filter: 595LP
    • Color name available on the instrument: BV605 
  • Detector: E
    • Band Pass Filter: 525/50
    • Dichoric Filter: 505LP
    • Color name available on the instrument: AmCyan
    • Additional Color: CFP/Sytox Blue/Fixable Aqua/BV510
  • Detector: F
    • Band Pass Filter: 450/50
    • Dichoric Filter: Blank
    • Color name available on the instrument: Pacific Blue
    • Additional Color: DAPI/Sytox Blue/Fixable Violet/V450/BV421

Red 633nm

  • Detector: A
    • Band Pass Filter: 780/60
    • Dichoric Filter: 750LP
    • Color name available on the instrument: APC-Cy7
    • Additional Color: Fixable Near IR/APC-H7/APC-A780
  • Detector: B
    • Band Pass Filter: 730/45
    • Dichoric Filter: 685LP
    • Color name available on the instrument: Alexa700
    • Additional Color: APC-R700
  • Detector: C
    • Band Pass Filter: 670/30
    • Dichoric Filter: Blank
    • Color name available on the instrument: APC
    • Additional Color: Sytox Red/Fixable Far Red/Alexa633 Alexa647/DRAQ5

BD LSRII (CCTI Site)

Blue 488nm

  • Detector: A
    • Band Pass Filter: 710/50
    • Dichoric Filter: 685LP
    • Color name available on the instrument: PerCP-Cy5.5/PE-Cy5
    • Additional Color: PI
    • Channel in Fortessa: Yes
  • Detector: B 
    • Band Pass Filter: 525/50
    • Dichoric Filter: 505LP
    • Color name available on the instrument: FITC/GFP/Alexa488
    • Additional Color: Sytox Green/Fixable Green
    • Channel in Fortessa: Yes
  • Detector: C
    • Band Pass Filter: 488/10
    • Dichoric Filter: Blank
    • Color name available on the instrument: SSC
    • Channel in Fortessa: Yes

Green 532nm

  • Detector: A
    • Band Pass Filter: 780/60
    • Dichoric Filter: 750LP
    • Color name available on the instrument: PE-Cy7
    • Channel in Fortessa: Yes
  • Detector: C
    • Band Pass Filter: 610/20
    • Dichoric Filter: 600LP
    • Color name available on the instrument: PE-Texas Red/PE-CF594
    • Additional Color: 7AAD/PI/Fixable Red
    • Channel in Fortessa: Yes
  • Detector: D
    • Band Pass Filter: 575/25
    • Dichoric Filter: Blank
    • Color name available on the instrument: PE
    • Additional Color: Sytox Orange/PI
    • Channel in Fortessa: Yes

Violet 405nm

  • Detector: A
    • Band Pass Filter: 710/50
    • Dichoric Filter: 685LP
    • Color name available on the instrument: Qdot705
    • Additional Color: BV711
    • Channel in Fortessa: Yes
  • Detector: B
    • Band Pass Filter: 655/12
    • Dichoric Filter: 635LP
    • Color name available on the instrument: Qdot655
    • Additional Color: BV650
    • Channel in Fortessa: Yes
  • Detector: C
    • Band Pass Filter: 610/20
    • Dichoric Filter: 595LP
    • Color name available on the instrument: Qdot605
    • Additional Color: BV605
    • Channel in Fortessa: Yes
  • Detector: D
    • Band Pass Filter: 515/30
    • Dichoric Filter: 505LP
    • Color name available on the instrument: Alexa430/AmCyan/Pac Orange/V500/ (BV570 filter change)
    • Additional Color: CFP/Sytox Blue/Fixable Aqua/BV510
    • Channel in Fortessa: Yes
  • Detector: E
    • Band Pass Filter: 450/50
    • Dichoric Filter: Blank
    • Color name available on the instrument: Alexa405/Pac Blue/Cascade Blue/V450/BV421
    • Additional Color: DAPI/Sytox Blue/Fixable Violet
    • Channel in Fortessa: Yes

Red 633nm

  • Detector: A
    • Band Pass Filter: 780/60
    • Dichoric Filter: 750LP
    • Color name available on the instrument: APC-Cy7/APC-H7/APC-A780
    • Additional Color: Fixable Near IR
    • Channel in Fortessa: Yes
  • Detector: B 
    • Band Pass Filter: 730/45
    • Dichoric Filter: 685LP
    • Color name available on the instrument: Alexa700
    • Additional Color: APC-R700
    • Channel in Fortessa: Yes
  • Detector: C
    • Band Pass Filter: 670/30
    • Dichoric Filter: Blank
    • Color name available on the instrument: Alexa647/APC/DRAQ5
    • Additional Color: Sytox Red/Fixable Far Red/Alexa633
    • Channel in Fortessa: Yes

UV 355nm

  • Detector: A
    • Band Pass Filter: 620/10
    • Dichoric Filter: 610LP
    • Color name available on the instrument: Qdot605UV
    • Additional Color: Hoe Red/(BUV737 filter change)
    • Channel in Fortessa: No
  • Detector: B
    • Band Pass Filter: 515/30
    • Dichoric Filter: 505LP
    • Color name available on the instrument: Indo-1 Blue/Qdot 525
    • Additional Color: live/dead aqua/BUV496(DAPI filter change)
    • Channel in Fortessa: No
  • Detector: C
    • Band Pass Filter: 450/50
    • Dichoric Filter: Blank
    • Color name available on the instrument: DAPI/Alexa350/Indo-1 violet/
    • Additional Color: Fixable Blue/Hoe Blue/ (BUV395 filter change)
    • Channel in Fortessa: No

Orange 594nm

  • Detector: A
    • Band Pass Filter: 685/35
    • Dichoric Filter: 670LP
    • Color name available on the instrument: mPlum
    • Channel in Fortessa: No
  • Detector: B
    • Band Pass Filter: 620/20
    • Dichoric Filter: Blank
    • Color name available on the instrument: Alexa594/568, mCherry, mStraw, Texas Red
    • Channel in Fortessa: No

BD LSRII (HICCC Site)

Blue 488nm

  • Detector: A
    • Band Pass Filter: 780/60
    • Dichoric Filter: 735LP
    • Color name available on the instrument: PE-Cy7
  • Detector: B
    • Band Pass Filter: 695/40
    • Dichoric Filter: 635LP
    • Color name available on the instrument: PerCP
    • Additional Color: PerCP-Cy5.5/PE-Cy5/7AAD
  • Detector: C
    • Band Pass Filter: 610/20
    • Dichoric Filter: 600LP
    • Color name available on the instrument: PE-TR
    • Additional Color: PE-CF594/PE-Dazzle594/PI
  • Detector: D
    • Band Pass Filter: 575/26
    • Dichoric Filter: 550LP
    • Color name available on the instrument: PE
    • Additional Color: PI
  • Detector: E
    • Band Pass Filter: 525/50
    • Dichoric Filter: 505LP
    • Color name available on the instrument: FITC/Alexa488
    • Additional Color: GFP/Sytox Green/Fixable Green
  • Detector: F
    • Band Pass Filter: 488/10
    • Dichoric Filter: Blank
    • Color name available on the instrument: SSC

Violet 405nm

  • Detector: A
    • Band Pass Filter: 780/60
    • Dichoric Filter: 750LP
    • Color name available on the instrument: BV786
  • Detector: B
    • Band Pass Filter: 710/50
    • Dichoric Filter: 690LP
    • Color name available on the instrument: BV711
  • Detector: C
    • Band Pass Filter: 670/30
    • Dichoric Filter: 635LP
    • Color name available on the instrument: BV650
  • Detector: D
    • Band Pass Filter: 610/20
    • Dichoric Filter: 595LP
    • Color name available on the instrument: 
    • Additional Color: BV605
  • Detector: E
    • Band Pass Filter: 525/50
    • Dichoric Filter: 505LP
    • Color name available on the instrument: BV510
    • Additional Color: AmCyan/V500/CFP/Sytox Blue/Fixable Aqua/BV510
  • Detector: F
    • Band Pass Filter: 450/50
    • Dichoric Filter: Blank
    • Color name available on the instrument: BV421
    • Additional Color: Pac Blue/Cascade Blue/V450

Red 633nm

  • Detector: A
    • Band Pass Filter: 780/60
    • Dichoric Filter: 735LP
    • Color name available on the instrument: APC-Cy7
    • Additional Color: Fixable Near IR/APC-H7/APC-A780
  • Detector: B
    • Band Pass Filter: 710/20
    • Dichoric Filter: 685LP
    • Color name available on the instrument: Alexa700
    • Additional Color: APC-R700
  • Detector: C
    • Band Pass Filter: 660/20
    • Dichoric Filter: Blank
    • Color name available on the instrument: APC
    • Additional Color: Alexa647/Sytox Red/Fixable Far Red/Alexa633/DRAQ5

UV255nm

  • Detector: A
    • Band Pass Filter: 740/35
    • Dichoric Filter: 690LP
    • Color name available on the instrument: BUV737
  • Detector: B
    • Band Pass Filter: 530/30
    • Dichoric Filter: 505LP
    • Color name available on the instrument: BUV496
    • Additional Color: live/dead aqua/BUV496/Indo-1Blue
  • Detector: C
    • Band Pass Filter: 450/40
    • Dichoric Filter: 410LP
    • Color name available on the instrument: DAPI
    • Additional Color: Fixable Blue/Hoe Blue/Alexa350/Indo-1 violet
  • Detector: D
    • Band Pass Filter: 379/28
    • Dichoric Filter: Blank
    • Color name available on the instrument: BUV395

BD FACSCantoII (CCTI Site)

Blue 488nm

  • Detector: A
    • Band Pass Filter: 780/60
    • Dichoric Filter: 735LP
    • Color: PE-Cy7
  • Detector: B
    • Band Pass Filter:  670LP
    • Dichoric Filter: 655LP
    • Color: PerCP-Cy5.5/PerCP/7-AAD
  • Detector: C
    • Band Pass Filter: No PMT
    • Dichoric Filter: 610LP
    • Color: None
  • Detector: D
    • Band Pass Filter: 585/42
    • Dichoric Filter: 556LP
    • Color: PE/PI
  • Detector: E
    • Band Pass Filter: 530/30
    • Dichoric Filter: 502LP
    • Color: FITC/GFP/Alexa488
  • Detector: F
    • Band Pass Filter: 488/10
    • Dichoric Filter: Blank
    • Color: SSC

Violet 405nm

  • Detector: A
    • Band Pass Filter: 510/50
    • Dichoric Filter: 502LP
    • Color: PacOrange/Alexa430/AmCyan/CFP/V500/BV510
  • Detector: B
    • Band Pass Filter: 450/50
    • Dichoric Filter: Blank
    • Color: Pac Blue/Alexa405/V450/DAPI/BV421

Red 633nm

  • Detector: A
    • Band Pass Filter: 780/60
    • Dichoric Filter: 735LP
    • Color: APC-Cy7/APC-H7
  • Detector: B
    • Band Pass Filter: No PMT
    • Dichoric Filter: 685LP
    • Color: None
  • Detector: C
    • Band Pass Filter: 660/30
    • Dichoric Filter: Blank
    • Color: Alexa633/Alexa647/APC/DRAQ5

BB Influx (P&S17-514) and P&S Influx (P&S 17-503) (CCTI Site)

Blue 488nm

  • Detector: A 
    • Band Pass Filter: 488/10
    • Color: SSC
  • Detector: B
    • Band Pass Filter: 530/40
    • Live/Dead: Sytox Green
    • Fixable: Green
    • Color: FITC/A488/GFP
  • Detector: C
    • Band Pass Filter: 692/40
    • Live/Dead: 7AAD/PI
    • Color: PerCP-Cy5.5

Green 561nm

  • Detector: A
    • Band Pass Filter: 585/29
    • Live/Dead: Sytox Orange
    • Color: PE
  • Detector: B
    • Band Pass Filter: 610/20
    • Live/Dead: 7AAD/PI
    • Fixable: Red
    • Color: PE-Texas Red
  • Detector: C
    • Band Pass Filter: 670/30
    • Live/Dead: 7AAD/PI
    • Color: PE-Cy5
  • Detector: D
    • Band Pass Filter: 750LP
    • Color: PE-Cy7

Violet 405nm

  • Detector: A 
    • Band Pass Filter: 460/50
    • Live/Dead: DAPI/Sytox Blue
    • Fixable: Violet
    • Color: PacBlue/V450/BV421
  • Detector: B
    • Band Pass Filter: 520/35
    • Live/Dead: Sytox Blue
    • Fixable: Aqua
    • Color: AmCyan/PacOrange/V500/Q525/BV510
  • Detector: C
    • Band Pass Filter: 610/20
    • Color: Q605/BV605
  • Detector: D
    • Band Pass Filter: 660/20
    • Color: Q655/BV650
  • Detector: E
    • Band Pass Filter: 710/50
    • Color: Q705/BV711
  • Detector: F
    • Band Pass Filter: 750LP
    • Color: Q800/BV785

Red 638nm

  • Detector: A
    • Band Pass Filter: 670/30
    • Live/Dead: Sytox Red
    • Fixable: Far Red
    • Color: APC/A633/A647
  • Detector: B
    • Band Pass Filter: 720/40
    • Color: A700
  • Detector: C 
    • Band Pass Filter: 750LP
    • Fixable: Near IR
    • Color: APC-Cy7/APC-H7

Aria II (HICCC Site)

Blue 488nm

  • Detector: A
    • Band Pass Filter: 695/40
    • Dichoric Filter: 600LP
    • Color name available on the instrument: PerCP-Cy5.5
  • Detector: B
    • Band Pass Filter: 530/30
    • Dichoric Filter: 505LP
    • Color name available on the instrument: FITC
    • Additional Color: Sytox Green/Fixable Green/GFP/Alexa488
  • Detector: C
    • Band Pass Filter: 488/10
    • Dichoric Filter: Blank
    • Color name available on the instrument: SSC

Green 561nm

  • Detector: A
    • Band Pass Filter: 780/60
    • Dichoric Filter: 755LP
    • Color name available on the instrument: PE-Cy7
  • Detector: B
    • Band Pass Filter: 610/20
    • Dichoric Filter: 600LP
    • Color name available on the instrument: mCherry
    • Additional Color: 7AAD/PI/Fixable Red/PE-CF594/ PE-Texas Red
  • Detector: C
    • Band Pass Filter: 575/25
    • Dichoric Filter: Blank
    • Color name available on the instrument: PE
    • Additional Color: Sytox Orange/PI

Violet 405nm

  • Detector: A
    • Band Pass Filter: 780/60
    • Dichoric Filter: 755LP
    • Color name available on the instrument: BV786
  • Detector: B
    • Band Pass Filter: 710/50
    • Dichoric Filter: 685LP
    • Color name available on the instrument: BV711
  • Detector: C
    • Band Pass Filter: 660/20
    • Dichoric Filter: 630LP
    • Color name available on the instrument: BV655
  • Detector: D
    • Band Pass Filter: 610/20
    • Dichoric Filter: 595LP
    • Color name available on the instrument: BV605
  • Detector: E
    • Band Pass Filter: 525/50
    • Dichoric Filter: 505LP
    • Color name available on the instrument: BV510
    • Additional Color: CFP/Sytox Blue/Fixable Aqua/AmCyan
  • Detector: F
    • Band Pass Filter: 450/50
    • Dichoric Filter: Blank
    • Color name available on the instrument: BV421
    • Additional Color: DAPI/Sytox Blue/Fixable Violet/V450/ Pacific Blue

Red 633nm

  • Detector: A
    • Band Pass Filter: 780/60
    • Dichoric Filter: 755LP
    • Color name available on the instrument: APC-Cy7
    • Additional Color: Fixable Near IR/APC-H7/APC-A780
  • Detector: B
    • Band Pass Filter: 730/44
    • Dichoric Filter: 690LP
    • Color name available on the instrument: Alexa Fluor 700
    • Additional Color: APC-R700
  • Detector: C
    • Band Pass Filter: 670/30
    • Dichoric Filter: Blank
    • Color name available on the instrument: APC
    • Additional Color: Sytox Red/Fixable Far Red/Alexa633 Alexa647/DRAQ5

BD Influx (ICRC 301) (HICCC Site)

Blue 488nm

  • Detector: A
    • Band Pass Filter: 488/10
    • Color: SSC
  • Detector: B
    • Band Pass Filter: 530/40
    • Live/Dead: Sytox Green
    • Fixable: Green
    • Color: FITC/A488/GFP
  • Detector: C
    • Band Pass Filter: 692/40
    • Live/Dead: 7AAD/PI
    • Color: PerCP-Cy5.5

Green 561nm

  • Detector: A
    • Band Pass Filter: 585/29
    • Live/Dead: Sytox Orange
    • Color: PE
  • Detector: B
    • Band Pass Filter: 610/20
    • Live/Dead: 7AAD/PI
    • Fixable: Red
    • Color: PE-Texas Red
  • Detector: C
    • Band Pass Filter: 670/30
    • Live/Dead: 7AAD/PI
    • Color: PE-Cy5
  • Detector: D
    • Band Pass Filter: 750LP
    • Color: PE-Cy7

Violet 405nm

  • Detector: A
    • Band Pass Filter: 460/50
    • Live/Dead: DAPI/Sytox Blue
    • Fixable: Violet
    • Color: PacBlue/V450/BV421
  • Detector: B
    • Band Pass Filter: 520/35
    • Live/Dead: Sytox Blue
    • Fixable: Aqua
    • Color: AmCyan/PacOrange/V500/Q525/BV510
  • Detector: C
    • Band Pass Filter: 610/20
    • Color: Q605/BV605
  • Detector: D
    • Band Pass Filter: 660/20
    • Color: Q655/BV650
  • Detector: E
    • Band Pass Filter: 710/50
    • Color: Q705/BV711
  • Detector: F
    • Band Pass Filter: 750LP
    • Color: Q800/BV785

Red 638nm

  • Detector: A
    • Band Pass Filter: 670/30
    • Live/Dead: Sytox Red
    • Fixable: Far Red
    • Color: APC/A633/A647
  • Detector: B
    • Band Pass Filter: 720/40
    • Color: A700
  • Detector: C
    • Band Pass Filter: 750LP
    • Fixable: Near IR
    • Color: APC-Cy7/APC-H7

Leica DMI 6000B

DAP

Excitation Filter: 405/10

Emission Filter: 406/40

Color: DAPI/Hoechst

GFP

Excitation Filter: 470/40

Emission Filter: 525/50

Color: FITC/GFP/Alexa488

Cy3

Excitation Filter: 545/30

Emission Filter: 610/75

Color: PE/Alexa546/Alexa555/Cy3/Rhodamine B

Cy5

Excitation Filter: 620/60

Emission Filter: 700/75

Color: Alexa633/Alexa647/APC/Cy5